| Saturday, 6 September 2025 |
| Blackpool | v | Liverpool St Helens 2nd XV |
| Kendal 2nd XV | v | Sedgley Park 3rd XV |
| Littleborough 2nd XV | v | Fylde Vandals (4th XV) |
| Preston Grasshoppers 3rd XV | v | Southport 2nd XV |
| Tarleton 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Wigan 2nd XV | v | Aspull Development XV |
| Saturday, 13 September 2025 |
| Fylde Vandals (4th XV) | v | Blackpool |
| Kirkby Lonsdale RUFC Development XV | v | Kendal 2nd XV |
| Liverpool St Helens 2nd XV | v | Preston Grasshoppers 3rd XV |
| Sedgley Park 3rd XV | v | Littleborough 2nd XV |
| Southport 2nd XV | v | Aspull Development XV |
| Tarleton 2nd XV | v | Wigan 2nd XV |
| Saturday, 4 October 2025 |
| Aspull Development XV | v | Liverpool St Helens 2nd XV |
| Blackpool | v | Sedgley Park 3rd XV |
| Kendal 2nd XV | v | Tarleton 2nd XV |
| Littleborough 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Preston Grasshoppers 3rd XV | v | Fylde Vandals (4th XV) |
| Wigan 2nd XV | v | Southport 2nd XV |
| Saturday, 11 October 2025 |
| Fylde Vandals (4th XV) | v | Aspull Development XV |
| Kendal 2nd XV | v | Wigan 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Blackpool |
| Liverpool St Helens 2nd XV | v | Southport 2nd XV |
| Sedgley Park 3rd XV | v | Preston Grasshoppers 3rd XV |
| Tarleton 2nd XV | v | Littleborough 2nd XV |
| Saturday, 18 October 2025 |
| Aspull Development XV | v | Sedgley Park 3rd XV |
| Blackpool | v | Tarleton 2nd XV |
| Littleborough 2nd XV | v | Kendal 2nd XV |
| Preston Grasshoppers 3rd XV | v | Kirkby Lonsdale RUFC Development XV |
| Southport 2nd XV | v | Fylde Vandals (4th XV) |
| Wigan 2nd XV | v | Liverpool St Helens 2nd XV |
| Saturday, 8 November 2025 |
| Fylde Vandals (4th XV) | v | Liverpool St Helens 2nd XV |
| Kendal 2nd XV | v | Blackpool |
| Kirkby Lonsdale RUFC Development XV | v | Aspull Development XV |
| Littleborough 2nd XV | v | Wigan 2nd XV |
| Sedgley Park 3rd XV | v | Southport 2nd XV |
| Tarleton 2nd XV | v | Preston Grasshoppers 3rd XV |
| Saturday, 22 November 2025 |
| Aspull Development XV | v | Tarleton 2nd XV |
| Blackpool | v | Littleborough 2nd XV |
| Liverpool St Helens 2nd XV | v | Sedgley Park 3rd XV |
| Preston Grasshoppers 3rd XV | v | Kendal 2nd XV |
| Southport 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Wigan 2nd XV | v | Fylde Vandals (4th XV) |
| Saturday, 29 November 2025 |
| Blackpool | v | Wigan 2nd XV |
| Kendal 2nd XV | v | Aspull Development XV |
| Kirkby Lonsdale RUFC Development XV | v | Liverpool St Helens 2nd XV |
| Littleborough 2nd XV | v | Preston Grasshoppers 3rd XV |
| Sedgley Park 3rd XV | v | Fylde Vandals (4th XV) |
| Tarleton 2nd XV | v | Southport 2nd XV |
| Saturday, 6 December 2025 |
| Aspull Development XV | v | Littleborough 2nd XV |
| Fylde Vandals (4th XV) | v | Kirkby Lonsdale RUFC Development XV |
| Liverpool St Helens 2nd XV | v | Tarleton 2nd XV |
| Preston Grasshoppers 3rd XV | v | Blackpool |
| Southport 2nd XV | v | Kendal 2nd XV |
| Wigan 2nd XV | v | Sedgley Park 3rd XV |
| Saturday, 13 December 2025 |
| Blackpool | v | Aspull Development XV |
| Kendal 2nd XV | v | Liverpool St Helens 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Sedgley Park 3rd XV |
| Littleborough 2nd XV | v | Southport 2nd XV |
| Preston Grasshoppers 3rd XV | v | Wigan 2nd XV |
| Tarleton 2nd XV | v | Fylde Vandals (4th XV) |
| Saturday, 20 December 2025 |
| Aspull Development XV | v | Preston Grasshoppers 3rd XV |
| Fylde Vandals (4th XV) | v | Kendal 2nd XV |
| Liverpool St Helens 2nd XV | v | Littleborough 2nd XV |
| Sedgley Park 3rd XV | v | Tarleton 2nd XV |
| Southport 2nd XV | v | Blackpool |
| Wigan 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Saturday, 10 January 2026 |
| Aspull Development XV | v | Southport 2nd XV |
| Blackpool | v | Fylde Vandals (4th XV) |
| Kendal 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Littleborough 2nd XV | v | Sedgley Park 3rd XV |
| Preston Grasshoppers 3rd XV | v | Liverpool St Helens 2nd XV |
| Wigan 2nd XV | v | Tarleton 2nd XV |
| Saturday, 17 January 2026 |
| Fylde Vandals (4th XV) | v | Preston Grasshoppers 3rd XV |
| Kirkby Lonsdale RUFC Development XV | v | Littleborough 2nd XV |
| Liverpool St Helens 2nd XV | v | Aspull Development XV |
| Sedgley Park 3rd XV | v | Blackpool |
| Southport 2nd XV | v | Wigan 2nd XV |
| Tarleton 2nd XV | v | Kendal 2nd XV |
| Saturday, 24 January 2026 |
| Aspull Development XV | v | Fylde Vandals (4th XV) |
| Blackpool | v | Kirkby Lonsdale RUFC Development XV |
| Littleborough 2nd XV | v | Tarleton 2nd XV |
| Preston Grasshoppers 3rd XV | v | Sedgley Park 3rd XV |
| Southport 2nd XV | v | Liverpool St Helens 2nd XV |
| Wigan 2nd XV | v | Kendal 2nd XV |
| Saturday, 14 February 2026 |
| Fylde Vandals (4th XV) | v | Southport 2nd XV |
| Kendal 2nd XV | v | Littleborough 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Preston Grasshoppers 3rd XV |
| Liverpool St Helens 2nd XV | v | Wigan 2nd XV |
| Sedgley Park 3rd XV | v | Aspull Development XV |
| Tarleton 2nd XV | v | Blackpool |
| Saturday, 28 February 2026 |
| Aspull Development XV | v | Kirkby Lonsdale RUFC Development XV |
| Blackpool | v | Kendal 2nd XV |
| Liverpool St Helens 2nd XV | v | Fylde Vandals (4th XV) |
| Preston Grasshoppers 3rd XV | v | Tarleton 2nd XV |
| Southport 2nd XV | v | Sedgley Park 3rd XV |
| Wigan 2nd XV | v | Littleborough 2nd XV |
| Saturday, 7 March 2026 |
| Fylde Vandals (4th XV) | v | Wigan 2nd XV |
| Kendal 2nd XV | v | Preston Grasshoppers 3rd XV |
| Kirkby Lonsdale RUFC Development XV | v | Southport 2nd XV |
| Littleborough 2nd XV | v | Blackpool |
| Sedgley Park 3rd XV | v | Liverpool St Helens 2nd XV |
| Tarleton 2nd XV | v | Aspull Development XV |
| Saturday, 21 March 2026 |
| Aspull Development XV | v | Kendal 2nd XV |
| Fylde Vandals (4th XV) | v | Sedgley Park 3rd XV |
| Liverpool St Helens 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Preston Grasshoppers 3rd XV | v | Littleborough 2nd XV |
| Southport 2nd XV | v | Tarleton 2nd XV |
| Wigan 2nd XV | v | Blackpool |
| Saturday, 28 March 2026 |
| Blackpool | v | Preston Grasshoppers 3rd XV |
| Kendal 2nd XV | v | Southport 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Fylde Vandals (4th XV) |
| Littleborough 2nd XV | v | Aspull Development XV |
| Sedgley Park 3rd XV | v | Wigan 2nd XV |
| Tarleton 2nd XV | v | Liverpool St Helens 2nd XV |
| Saturday, 11 April 2026 |
| Aspull Development XV | v | Blackpool |
| Fylde Vandals (4th XV) | v | Tarleton 2nd XV |
| Liverpool St Helens 2nd XV | v | Kendal 2nd XV |
| Sedgley Park 3rd XV | v | Kirkby Lonsdale RUFC Development XV |
| Southport 2nd XV | v | Littleborough 2nd XV |
| Wigan 2nd XV | v | Preston Grasshoppers 3rd XV |
| Saturday, 18 April 2026 |
| Blackpool | v | Southport 2nd XV |
| Kendal 2nd XV | v | Fylde Vandals (4th XV) |
| Kirkby Lonsdale RUFC Development XV | v | Wigan 2nd XV |
| Littleborough 2nd XV | v | Liverpool St Helens 2nd XV |
| Preston Grasshoppers 3rd XV | v | Aspull Development XV |
| Tarleton 2nd XV | v | Sedgley Park 3rd XV |
| Saturday, 25 April 2026 |
| Aspull Development XV | v | Wigan 2nd XV |
| Fylde Vandals (4th XV) | v | Littleborough 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Tarleton 2nd XV |
| Liverpool St Helens 2nd XV | v | Blackpool |
| Sedgley Park 3rd XV | v | Kendal 2nd XV |
| Southport 2nd XV | v | Preston Grasshoppers 3rd XV |