| Saturday, 6 September 2025 |
| Blackpool | 12 | 17 | Liverpool St Helens 2nd XV |
| Kendal 2nd XV | 96 | 7 | Sedgley Park 3rd XV |
| Littleborough 2nd XV | 42 | 14 | Fylde Vandals (4th XV) |
| Preston Grasshoppers 3rd XV | 42 | 19 | Southport 2nd XV |
| Tarleton 2nd XV | Home Walkover | Kirkby Lonsdale RUFC Development XV |
| Wigan 2nd XV | 50 | 5 | Aspull Development XV |
| Saturday, 13 September 2025 |
| Fylde Vandals (4th XV) | 59 | 12 | Blackpool |
| Liverpool St Helens 2nd XV | 10 | 49 | Preston Grasshoppers 3rd XV |
| Sedgley Park 3rd XV | 21 | 5 | Littleborough 2nd XV |
| Southport 2nd XV | 74 | 0 | Aspull Development XV |
| Tarleton 2nd XV | 47 | 17 | Wigan 2nd XV |
| Saturday, 27 September 2025 |
| Kirkby Lonsdale RUFC Development XV | 20 | 42 | Kendal 2nd XV |
| Saturday, 4 October 2025 |
| Aspull Development XV | 31 | 5 | Liverpool St Helens 2nd XV |
| Kendal 2nd XV | 49 | 14 | Tarleton 2nd XV |
| Preston Grasshoppers 3rd XV | 10 | 27 | Fylde Vandals (4th XV) |
| Saturday, 11 October 2025 |
| Fylde Vandals (4th XV) | 46 | 19 | Aspull Development XV |
| Kirkby Lonsdale RUFC Development XV | 32 | 20 | Blackpool |
| Liverpool St Helens 2nd XV | 19 | 31 | Southport 2nd XV |
| Sedgley Park 3rd XV | 12 | 24 | Preston Grasshoppers 3rd XV |
| Tarleton 2nd XV | 43 | 24 | Littleborough 2nd XV |
| Saturday, 18 October 2025 |
| Aspull Development XV | 53 | 10 | Sedgley Park 3rd XV |
| Blackpool | 10 | 60 | Tarleton 2nd XV |
| Littleborough 2nd XV | 41 | 74 | Kirkby Lonsdale RUFC Development XV |
| Southport 2nd XV | 24 | 19 | Fylde Vandals (4th XV) |
| Wigan 2nd XV | 22 | 22 | Liverpool St Helens 2nd XV |
| Saturday, 25 October 2025 |
| Wigan 2nd XV | 21 | 17 | Southport 2nd XV |
| Saturday, 8 November 2025 |
| Fylde Vandals (4th XV) | 22 | 17 | Liverpool St Helens 2nd XV |
| Kendal 2nd XV | 79 | 0 | Blackpool |
| Kirkby Lonsdale RUFC Development XV | 34 | 0 | Aspull Development XV |
| Littleborough 2nd XV | 14 | 27 | Wigan 2nd XV |
| Sedgley Park 3rd XV | Away Walkover | Southport 2nd XV |
| Tarleton 2nd XV | 26 | 19 | Preston Grasshoppers 3rd XV |
| Saturday, 15 November 2025 |
| Blackpool | Home Walkover | Sedgley Park 3rd XV |
| Preston Grasshoppers 3rd XV | 21 | 29 | Kirkby Lonsdale RUFC Development XV |
| Saturday, 22 November 2025 |
| Aspull Development XV | 23 | 6 | Tarleton 2nd XV |
| Blackpool | 24 | 19 | Littleborough 2nd XV |
| Liverpool St Helens 2nd XV | 44 | 10 | Sedgley Park 3rd XV |
| Preston Grasshoppers 3rd XV | 12 | 87 | Kendal 2nd XV |
| Southport 2nd XV | 41 | 0 | Kirkby Lonsdale RUFC Development XV |
| Wigan 2nd XV | 33 | 17 | Fylde Vandals (4th XV) |
| Saturday, 29 November 2025 |
| Blackpool | v | Wigan 2nd XV |
| Kendal 2nd XV | v | Aspull Development XV |
| Kirkby Lonsdale RUFC Development XV | v | Liverpool St Helens 2nd XV |
| Littleborough 2nd XV | v | Preston Grasshoppers 3rd XV |
| Sedgley Park 3rd XV | v | Fylde Vandals (4th XV) |
| Tarleton 2nd XV | v | Southport 2nd XV |
| Saturday, 6 December 2025 |
| Aspull Development XV | v | Littleborough 2nd XV |
| Fylde Vandals (4th XV) | v | Kirkby Lonsdale RUFC Development XV |
| Liverpool St Helens 2nd XV | v | Tarleton 2nd XV |
| Preston Grasshoppers 3rd XV | v | Blackpool |
| Southport 2nd XV | v | Kendal 2nd XV |
| Wigan 2nd XV | v | Sedgley Park 3rd XV |
| Saturday, 13 December 2025 |
| Blackpool | v | Aspull Development XV |
| Kendal 2nd XV | v | Liverpool St Helens 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Sedgley Park 3rd XV |
| Littleborough 2nd XV | v | Southport 2nd XV |
| Preston Grasshoppers 3rd XV | v | Wigan 2nd XV |
| Tarleton 2nd XV | v | Fylde Vandals (4th XV) |
| Saturday, 20 December 2025 |
| Aspull Development XV | v | Preston Grasshoppers 3rd XV |
| Fylde Vandals (4th XV) | v | Kendal 2nd XV |
| Liverpool St Helens 2nd XV | v | Littleborough 2nd XV |
| Sedgley Park 3rd XV | v | Tarleton 2nd XV |
| Southport 2nd XV | v | Blackpool |
| Wigan 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Saturday, 10 January 2026 |
| Aspull Development XV | v | Southport 2nd XV |
| Blackpool | v | Fylde Vandals (4th XV) |
| Littleborough 2nd XV | v | Sedgley Park 3rd XV |
| Preston Grasshoppers 3rd XV | v | Liverpool St Helens 2nd XV |
| Wigan 2nd XV | v | Tarleton 2nd XV |
| Saturday, 17 January 2026 |
| Blackpool | v | Sedgley Park 3rd XV |
| Fylde Vandals (4th XV) | v | Preston Grasshoppers 3rd XV |
| Kirkby Lonsdale RUFC Development XV | v | Littleborough 2nd XV |
| Liverpool St Helens 2nd XV | v | Aspull Development XV |
| Southport 2nd XV | v | Wigan 2nd XV |
| Tarleton 2nd XV | v | Kendal 2nd XV |
| Saturday, 24 January 2026 |
| Aspull Development XV | v | Fylde Vandals (4th XV) |
| Blackpool | v | Kirkby Lonsdale RUFC Development XV |
| Littleborough 2nd XV | v | Tarleton 2nd XV |
| Preston Grasshoppers 3rd XV | v | Sedgley Park 3rd XV |
| Southport 2nd XV | v | Liverpool St Helens 2nd XV |
| Wigan 2nd XV | v | Kendal 2nd XV |
| Wigan 2nd XV | v | Kendal 2nd XV |
| Friday, 30 January 2026 |
| Kendal 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Saturday, 14 February 2026 |
| Fylde Vandals (4th XV) | v | Southport 2nd XV |
| Kendal 2nd XV | v | Littleborough 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Preston Grasshoppers 3rd XV |
| Liverpool St Helens 2nd XV | v | Wigan 2nd XV |
| Sedgley Park 3rd XV | v | Aspull Development XV |
| Tarleton 2nd XV | v | Blackpool |
| Saturday, 21 February 2026 |
| Kirkby Lonsdale RUFC Development XV | v | Southport 2nd XV |
| Littleborough 2nd XV | v | Kendal 2nd XV |
| Saturday, 28 February 2026 |
| Aspull Development XV | v | Kirkby Lonsdale RUFC Development XV |
| Blackpool | v | Kendal 2nd XV |
| Liverpool St Helens 2nd XV | v | Fylde Vandals (4th XV) |
| Preston Grasshoppers 3rd XV | v | Tarleton 2nd XV |
| Southport 2nd XV | v | Sedgley Park 3rd XV |
| Wigan 2nd XV | v | Littleborough 2nd XV |
| Saturday, 7 March 2026 |
| Fylde Vandals (4th XV) | v | Wigan 2nd XV |
| Kendal 2nd XV | v | Preston Grasshoppers 3rd XV |
| Littleborough 2nd XV | v | Blackpool |
| Sedgley Park 3rd XV | v | Liverpool St Helens 2nd XV |
| Tarleton 2nd XV | v | Aspull Development XV |
| Saturday, 21 March 2026 |
| Aspull Development XV | v | Kendal 2nd XV |
| Fylde Vandals (4th XV) | v | Sedgley Park 3rd XV |
| Liverpool St Helens 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Preston Grasshoppers 3rd XV | v | Littleborough 2nd XV |
| Southport 2nd XV | v | Tarleton 2nd XV |
| Wigan 2nd XV | v | Blackpool |
| Saturday, 28 March 2026 |
| Blackpool | v | Preston Grasshoppers 3rd XV |
| Kendal 2nd XV | v | Southport 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Fylde Vandals (4th XV) |
| Littleborough 2nd XV | v | Aspull Development XV |
| Sedgley Park 3rd XV | v | Wigan 2nd XV |
| Tarleton 2nd XV | v | Liverpool St Helens 2nd XV |
| Saturday, 11 April 2026 |
| Aspull Development XV | v | Blackpool |
| Fylde Vandals (4th XV) | v | Tarleton 2nd XV |
| Liverpool St Helens 2nd XV | v | Kendal 2nd XV |
| Sedgley Park 3rd XV | v | Kirkby Lonsdale RUFC Development XV |
| Southport 2nd XV | v | Littleborough 2nd XV |
| Wigan 2nd XV | v | Preston Grasshoppers 3rd XV |
| Saturday, 18 April 2026 |
| Blackpool | v | Southport 2nd XV |
| Kendal 2nd XV | v | Fylde Vandals (4th XV) |
| Kirkby Lonsdale RUFC Development XV | v | Wigan 2nd XV |
| Littleborough 2nd XV | v | Liverpool St Helens 2nd XV |
| Preston Grasshoppers 3rd XV | v | Aspull Development XV |
| Tarleton 2nd XV | v | Sedgley Park 3rd XV |
| Saturday, 25 April 2026 |
| Aspull Development XV | v | Wigan 2nd XV |
| Fylde Vandals (4th XV) | v | Littleborough 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Tarleton 2nd XV |
| Liverpool St Helens 2nd XV | v | Blackpool |
| Sedgley Park 3rd XV | v | Kendal 2nd XV |
| Southport 2nd XV | v | Preston Grasshoppers 3rd XV |